Bahan utama yang digunakan dalam pembuatan metil ester adalah minyak goreng bekas. Fraksinasi ester metil dilakukan dengan teknik beku ke cair yaitu ester metil sebanyak 20 ml dimasukkan ke dalam tabung reaksi lalu ditempatkan dalam freezer dengan suhu 20. Konsep dasar kimia untuk pgsd 228 9 3.
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